In this project, a nonlinear optical microscopy utilizing two-photon energized fluorescence and second-symphonious age was utilized for the discovery of premalignant gastric injuries. It was discovered that organ morphology and collagen structure in mucosa will change with the movement of gastric maladies from ordinary to intestinal metaplasia, to poor quality intraepithelial neoplasia, and to high-review intraepithelial neoplasia, and this microscopy was capable to specifically recognize these notice indications.
Moreover, two highlights were evaluated from nonlinear optical pictures to illustrate the progressions of organ size and collagen content amid the improvement procedure of preneoplastic injuries. These outcomes obviously demonstrate that nonlinear optical microscopy can successfully separate typical and precancerous gastric tissues without differentiate operators, which would be useful for early conclusion and treatment of gastric infections. This examination may give the basis to further use of nonlinear optical microscopy in clinical practice.